Product Description
DL-Dithiothreitol (DTT), also famously known as Cleland’s reagent, is a powerful and versatile reducing agent essential for molecular biology, biochemistry, and proteomics. With CAS number 3483-12-3, this small-molecule redox reagent effectively reduces disulfide bonds in proteins and peptides, protecting them from intramolecular and intermolecular disulfide bond formation. Its ability to maintain sulfhydryl groups in a reduced state makes DTT a standard component in laboratory buffers used for protein extraction, purification, and analysis.
Product Properties
Property Value IUPAC Name (2R,3S)-1,4-bis(sulfanyl)butane-2,3-diol Synonym DTT, Cleland’s Reagent CAS Number 3483-12-3 Chemical Formula Molar Mass 154.25 g/mol Appearance White crystalline powder or solid Purity Typically ≥99% Solubility Highly soluble in water, ethanol, and acetone. Product Usage
- Protein Analysis: Widely used in sample buffers for SDS-PAGE to reduce protein disulfide bonds, ensuring accurate molecular weight determination.
- Enzyme Stabilization: Added to enzyme preparations and assay buffers to preserve enzymatic activity by preventing the oxidation of critical sulfhydryl groups.
- Protein Purification: Included in lysis and purification buffers (e.g., for chromatography) to maintain protein solubility and functionality.
- Molecular Cloning: Used in certain DNA-related applications, such as in some transcription buffers or to remove inhibitors from DNA preparations.
- Antioxidant Reagent: Acts as a general protective agent in vitro to prevent oxidative damage to biological molecules during experiments.
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